Which of the following is the first event to take place in translation in eukaryotes Quizlet

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Which of the following statements best describes the termination of transcription in prokaryotes?

RNA polymerase transcribes through the terminator sequence, causing the polymerase to separate from the DNA and release the transcript.

RNA polymerase transcribes through the polyadenylation signal, causing proteins to associate with the transcript and cut it free from the polymerase.

Once transcription has initiated, RNA polymerase transcribes until it reaches the end of the chromosome.

RNA polymerase transcribes through an intron, and the snRNPs cause the polymerase to let go of the transcript.

RNA polymerase transcribes through a stop codon, causing the polymerase to stop advancing through the gene and release the mRNA.

The trp operon is a repressible operon and it is negatively controlled by the level of its product, tryptophan. The trp operon regulatory gene trpR codes for a protein called trp repressor. Trp repressor protein is made as an inactive negative regulator. Two tryptophan molecules can bind to the dimeric trp repressor protein, changing its conformation to active DNA-binding conformation and its binding to the operator. The operator region overlaps with the promoter sequence. As a result, when the trp repressor binds to the operator it precludes the RNA polymerase binding to the overlapping promoter. When the level of the coregulator tryptophan is high in the cell, tryptophan binds to the trp repressor protein and activates it, thereby preventing the transcription of the trp operon. Once the level of tryptophan goes down, tryptophan molecules dissociate from the trp repressor making it unable to bind the operator, thus, initiating the transcription of the trp operon by RNA polymerase.

Attenuation is the second level of control in the trp operon. Attenuation allows to monitor the second pool of tryptophan, that of Trp-tRNA. The mRNA of the trp operon has a 5' leader sequence called the attenuator which contains a small ORF which codes for a small leader peptide of 14 amino acids. This ORF contains a ribosome-binding site whose AUG is followed by a short coding region which contains two successive codons for tryptophan. The translation of this leader sequence will decide the transcription termination or no termination of the trp operon. When the level of trp-tRNA is low, the ribosome will be stalled when it reach the tryptophan codon in the small ORF and influence the termination at the attenuator.

The leader sequence can be divided into four as region 1, 2, 3, and 4 which can base pair among themselves forming hairpin structures. This region is followed by a short U8 sequence. The position of the ribosome in the small ORF which lies in the region 1 will affect the base pairing of the regions.

Sets with similar terms

What is the very first step in the initiation of translation in eukaryotes quizlet?

What is the first step during the initiation of translation? -once mRNA is bound to the small subunit, an initiator aminoacetyl tRNA binds to the start codon on the mRNA, then the large subunit binds to the small subunit to complete the initiation complex!

Which of the following is first event to take place in translation in eukaryotes?

Translation is generally divided into three stages: initiation, elongation, and termination (Figure 7.8). In both prokaryotes and eukaryotes the first step of the initiation stage is the binding of a specific initiator methionyl tRNA and the mRNA to the small ribosomal subunit.

What is the first step of the translation phase?

Aminoacylation of tRNA is the First Step in Translation. It's also known as tRNA activation or charge. In the presence of ATP, amino acids are activated and connected to a particular tRNA.

What is the first event of translation?

Initiation ("beginning"): in this stage, the ribosome gets together with the mRNA and the first tRNA so translation can begin. Elongation ("middle"): in this stage, amino acids are brought to the ribosome by tRNAs and linked together to form a chain.