Which of the following molecules helps to hold the DNA strands apart while they are being replicated quizlet?

For a science fair project, two students decided to repeat the Hershey and Chase experiment, with modifications. They decided to radioactively label the nitrogen of the DNA, rather than the phosphate. They reasoned that each nucleotide has only one phosphate and two to five nitrogen atoms. Thus, labeling the nitrogen atoms would provide a stronger signal than labeling the phosphates. Why won't this experiment work?

A) Radioactive nitrogen has a half-life of 100,000 years, and the material would be too dangerous for too long.

B) Amino acids (and thus proteins) also have nitrogen atoms; thus, the radioactivity would not distinguish between DNA and proteins.

C) There is no radioactive isotope of nitrogen.

D) Although there are more nitrogens in a nucleotide, labeled phosphates actually have 16 extra neutrons; therefore, they are more radioactive.

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The spontaneous loss of amino groups from adenine in DNA results in hypoxanthine, an uncommon base, opposite thymine. What combination of proteins could repair such damage?
-nuclease, DNA polymerase, DNA ligase
-telomerase, helicase, single-strand binding protein
-nuclease, telomerase, primase
-telomerase, primase, DNA polymerase
-DNA ligase, replication fork proteins, adenylyl cyclase

In E. coli, to repair a thymine dimer by nucleotide excision repair, in which order do the necessary enzymes act?
-nuclease, DNA polymerase, RNA primase
-helicase, DNA polymerase, DNA ligase
-nuclease, DNA polymerase, DNA ligase
-DNA ligase, nuclease, helicase

To repair a thymine dimer by nucleotide excision repair, in which order do the necessary enzymes act?

A) exonuclease, DNA polymerase 3, RNA primase
B) helicase, DNA polymerase 1, DNA ligase
C) DNA ligase, nuclease, helicase
D) DNA polymerase 1, DNA polymerase 3, DNA ligase
E) endonuclease, DNA polymerase 1, DNA ligase

Which of the following sets of materials are required by both eukaryotes and prokaryotes for replication?

A) double-stranded DNA, four kinds of dNTPs, primers, origins
B) topoisonmerases, telomerases, polymerases
C) G-C rich regions, polymerases, chromosome nicks
D) nucleosome loosening, four dNTPs, four rNTPs
E) ligase, primers, mucleases

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Which molecules help to hold the DNA strands apart while they are being replicated?

Proteins called Binding Proteins keep the two DNA strands apart preserving the Replication Fork. A protein called Primase makes a short segment of RNA called the RNA Primer on the DNA termed the Template DNA.

What binds to the DNA strands to keep them separate?

DNA Helicases - These proteins bind to the double stranded DNA and stimulate the separation of the two strands. DNA single-stranded binding proteins - These proteins bind to the DNA as a tetramer and stabilize the single-stranded structure that is generated by the action of the helicases.

What bonds hold DNA strands together quizlet?

The two strands of DNA are bound together by hydrogen bonds which are present between the nitrogenous bases.

What holds DNA open?

Two DNA polymerase molecules work at the fork, one on the leading strand and one on the lagging strand. The DNA helix is opened by a DNA polymerase molecule clamped on the leading strand, acting in concert with one or more DNA helicase molecules running along the strands in front of it.